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Sequences from the ends of bacterial artificial chromosome (BAC) clones provide highly specific markers. A whole genome sequencing approach has been described in a map-as-you-go strategy. The complete sequence of a seed BAC is searched against a BAC end database and the minimally overlapping clones in each direction are selected for sequencing. As coverage increases, BAC end sequences provide samples for whole genome survey.
~743,000 end sequences from 470,000 clones (20 X clone coverage and 12% sequence coverage) have been generated by TIGR, UofWashington and CalTech, providing a sequence marker every 5 kb across the genome. The coverage by paired-ends on chromosome 22 is over 5X. The project is funded by DOE.
BAC Libraries
CIT978SK A B,
CIT-HSP C D1 D2,
RPCI-11
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BAC End Sequencing Protocals
Template,
Reaction,
Clean-Up
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Database Composition, see README
TIGR,
UofWash,
CalTech
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BAC End Search
By sequence,
By clone_name
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BAC End Annotation
Repeats, ESTs and STSs,
FISH,
phred quality
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BAC End FTP site
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Clone Availability
Research Genetics,
Genome Systems Inc,
Pieter de Jong
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BAC Related Links
Rat ends,
Mouse ends,
Chr 16,
CalTech,
Stanford RH,
UWash FISH,
Genome Channel,
GDB,
RPCI-11 fingerprint,
RPCI-11 screening,
RPCI,
NCBI Genome Guide,
CMSC FISH
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Please direct comments and inquiries on human BAC ends to Shaying Zhao
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