Pine Microarray Protocols

We have modified methods in use with the TIGR Potato Functional Genomics project for use with our pine cDNA arrays. For a complete listing of the potato protocols see: http://www.tigr.org/tdb/potato/microarray_SOPs.shtml.

The potato protocols that we are currently using on the pine cDNA array project are listed below.

• PCR Amplification Methods
  • 1.1.1 PCR amplification of cDNA inserts

• Array Printing Methods
  • 2.1.1 Array Printing

• RNA Labeling Methods
  • 5.2.2 Indirect labeling of RNA

• Hybridization Methods
  • 6.2.4 Hybridization using indirectly labeled probes

• mRNA Amplification and Hybridization
  • 9.1.1 mRNA Amplification and Hybridization

The NSF Genomics of Loblolly Pine Embryogenesis Project at IPST and TIGR is funded by the National Science Foundation.