We have started a project aimed at using sequence conservation between Arabidopsis and Brassica to enhance our annotation of the Arabidopsis genome. One part of the project, in collaboration with the Cold Spring Harbor group, is to perform whole genome shotgun sequencing with a goal of reaching 0.5 - 1x coverage of the Brassica genome. Alignment of these sequences with the finished Arabidopsis genome identifies conserved regions outside currently annotated gene boundaries that represent potential novel genes (both protein-coding and structural RNAs).

In parallel we are fully sequencing six Brassica BAC contigs identified by O'Neill and Bancroft (The Plant Journal 23: 233, 2000) as being homeologous to a region of the Arabidopsis genome that is duplicated between chromosomes 4 and 5. This will permit an in-depth comparison of homeologous regions both within and between Brassica and Arabidopsis and provide a more rigorous basis for interpreting the alignments between the Brassica shotgun sequences and Arabidopsis genomic DNA.

The B. oleracea genome sequence and annotation will be released on this web site and submitted to the public databases upon completion of the project. In the meantime, a BLAST server allows retrieval of sequences from the preliminary contigs. Please read the data release policy regarding the use of B. oleracea sequence data obtained from this site.

• What's New  Latest News on TIGR's Brassica oleracea sequencing effort.

• Sequence Search Provides searching of nucleotide or peptide sequences against preliminary contigs.

• Data Release Policy 

• Links  Links to Information related to the Brassica oleracea sequencing project available from other sites.