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BAC Tiling Path

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Sequences from the ends of bacterial artificial chromosome (BAC) clones provide highly specific markers. A whole-genome approach has been described to use BAC end sequences in a map-as-you-go strategy for genome sequencing. In this strategy , the complete sequence of a seed BAC is searched against a database of end-sequences from a BAC library to select the minimally overlapping clones to be sequenced next in each direction.

The current database includes end sequences generated at TIGR, CNS in France, and Salk. CNS sequences are taken from the CNS ftp site, whilst UPenn sequences are taken from dbGSS, trimmed according to the reported high quality coordinates in the dbGSS entry, and then masked for vector sequence. Click here for more information on the current BAC ends database.

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Read sequence from local file:
lastn search parameter s= 200 300 400 500 600
Minimum Percent Identity: 95 93 91 85
Percent Similarity: 97 95 93


Find BAC end sequences for particular BAC clones
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