Sequences from the ends of bacterial artificial chromosome (BAC) clones provide highly
specific markers. A whole-genome approach has been
described
to use BAC end sequences in a map-as-you-go strategy for genome sequencing. In this strategy
, the complete sequence of a seed BAC is searched against
a database of end-sequences from a BAC library to select the minimally overlapping clones to be
sequenced next in each direction.
The current database includes end sequences generated at TIGR, CNS
in France, and UPenn. CNS sequences
are taken from the CNS ftp site, whilst UPenn sequences are taken from
dbGSS, trimmed according to the reported high quality coordinates in the
dbGSS entry, and then masked for vector sequence. Click here for more
information on the current BAC ends database.