Frequently Asked Questions about EST and BAC clones

There is a separate FAQ page for all TIGR Gene Indices.

Can I order an EST clone from you?

TIGR does not own the EST clones, we simply obtained the sequence from public domains to build the gene index (AtGI). You can order a clone from the Ohio State Stock Center.

If you get the EST information by searching our site, you can order the clone from the stock center by following the link in the EST report returned. If a clone is available for an EST, the link will be provided for you in the EST report.

Sequence started with a name ET| is the transcript sequences for a characterized gene in GenBank (not an EST). The clones for those sequences are not all available from the stock center. You may want to follow the links to GenBank and contact the original authors about clone availability.

Can I order a BAC from you?

The easiest place to obtain a BAC clone would be from the Ohio State Stock Center. You can order individual clones online from them by using clone name as catalog number.

A BAC end sequence matched my favorite gene. Do you know where it is mapped to?

BAC end sequences are generated systematically from the TAMU and IGF libraries, in most cases without knowledge of their map position. In fact, most of the BAC clones have not been mapped as we generated end sequence from the entire library of BAC clones. Unless the sequence matches existing genomic sequence, we would have no further information on the map position.

However, there are two resources that may be of use to you in your search. Washington University at St Louis has fingerprinted many BAC clones and placed them into contigs. In addition Thomas Altmann's lab in Germany has produced contigs of clones based on hybridization data. Some of these contigs are anchored to mapped markers, and thus you may be able to determine its map position.

I saw a BAC end sequence from your site. When are you going to fully sequence the clone?

Only a small fraction of the BAC clones (10X of the genome) you see in BAC end database will be fully sequenced by the AGI labs. AtDB has a page tracking the status of the clones (BACs, P1, TAC, etc) selected for sequencing by all AGI labs. You can check there to see if a clone is being sequenced.

For Arabidopsis Comments/Questions send mail to at@tigr.org.

Introduction | Arabidopsis Genome Annotation Database (AGAD)
TIGR BAC Sequencing | Arabidopsis Gene Index (AtGI)
BAC End Sequence Database | FAQ | Arabidopsis Links